Abstract

DNA DAMAGE PROTECTIVE AND ANTI-INFLAMMATORY ACTIVITY OF MADHUCA INDICA AGAINST ELECTRON BEAM RADIATION

Vinutha K, Vidya Shimoga Muddappa*, Sujata Barangi, Suchetha Kumari N, Ganesh Sanjeev, Pradeepa, Vaman Rao C

ABSTRACT

Ionizing radiation is an important genotoxic agent causes deleterious effect to living system by generating free radicals produced via radical oxygen species (ROS). Protecting against free radicals especially by a natural plant source has several potential applications. In the present study, we have examined the ability of Madhuca indica (M. indica) ethanol stem bark extract, to inhibit radiation-induced DNA damage and inflammatory mediators. Our study showed that there was protection of pBR322 DNA (in vitro) upon exposure to 4 Gy electron beam (EB) radiation at different dose rate. Whole body exposure of mouse to EB radiation cause strand breaks in the cellular DNA, as observed using the comet assay and also there was substantial increase in the levels of inflammatory mediators. When animals exposed to radiation there was an increase in the percentage of DNA in tail (%T), tail length (TL) and olive tail moment (OTM). The presence of 400 mg/kg body weight of M. indica extract prior to irradiation significantly reduced comet parameters. Simultaneously the levels of nitric oxide (NO), malondialdehyde (MDA), and sialic acid concentration were reduced significantly when compared to irradiate, contributing to the overall radioprotective and anti-inflammatory activity of M. indica extract.

Keywords: Madhuca indica; MDA; NO; sialic acid; pBR322; EB radiation.