Abstract

METHOD DEVELOPMENT AND VALIDATION OF QUANTITATIVE ANALYTICAL METHOD FOR MOXIFLOXACIN AND KETOROLAC COMBINATION IN PHARMACEUTICAL DOSAGE FORM BY RP-HPLC

Priyadarshani S Bansode*, Chetan Singh Chauhan, Ravindra Kamble, Preeti Gopaliya, Chatrapal Singh

ABSTRACT

RP-HPLC is a physical separation technique conducted in the liquid phase in which a sample is separated into its constituent components (or analytes) by distributing between the mobile phase (a flowing liquid) and a stationary phase (sorbents packed inside a column). Prepare a degassed mixture of buffer and acetonitrile in the Ratio (60:40). Drugs showed maximum absorbance at 293 nm for Moxifloxacin and 245 nm for Ketorolac. Moxifloxacin is a broad-spectrum antibiotic that is active against both Gram-positive and Gram-negative bacteria. A nonsteroidal anti-inflammatory drug available as the tromethamine salt; used systemically for short-term management of pain. The proposed analytical method development and simultaneous estimation of Moxifloxacin and ketorolac and its validation by RP-HPLC is accurate, precise, simple, selective, sensitive and rapid can be applied successfully for routine analysis in quality control. The validation protocols usually followed is that defined by the International Conference on Harmonization (ICH) under their Validation for analytical procedures methodology.

Keywords: Moxifloxacin, Ketorolac, Reversed phase HPLC (RP-HPLC), validation.