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Abstract

DEVELOPMENT OF HPTLC METHOD FOR ESTIMATION OF VASICINE IN HERBAL FORMULATIONS AND QUALITY STANDARDIZATION IN ASFRE12

P. Ravi Kumar*

ABSTRACT

Vasicine is one of the phytoconstituents present in Adhatoda vasica (L.)Nees (Family: Acanthaceae) have been used in Indian systems of Medicine for cough, bronchitis, bronchial asthma etc. In this present study an attempt has been made to develop a HPTLC method for quantitative estimation of Vasicine in different extracts (Alkaloid extract, Methanolic extract and 70% Methanolic extract) and in different samples of Asfre12[Laboratory sample (Alkaloid extract), Commercial sample (Alkaloid extract), Laboratory sample (70% Methanolic extract), and Commercial sample (70% Methanolic extract)]. In this the alkaloid extract of A.vasica sample and laboratory sample of alkaloid extract yielded more percentage of Vasicine. Quality control and standardization was carried out in the polyherbal formulation (PHF) Asfre12.

Keywords: Adhatoda vasica, Vasicine, HPTLC, PHF, Asfre12.


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