Abstract

ANALYTICAL INVESTIGATION ON IRON (III) IN PRESENCE OF SURFACE SOILS, BIOLOGICAL SAMPLES, FOOD MATERIALS AND PHARMACEUITICAL SAMPLES USING 2-HYDROXY-3-METHOXY BENZALDEHYDE-p- HYDROXY BENZOIC HYDRAZONE (HMBAHBH)

G. Govindu*, V. Krishna Reddy and P. Raveendra Reddy

ABSTRACT

A new novel chromogenic reagent 2-hydroxy-3-methoxy benzaldehyde-p-hydroxy benzoic hydrazone (HMBAHBH) has been used for the direct spectrophotometric determination of Iron (III) in acidic surfactant of Triton X-100. HMBAHBH react with Fe(III) to form brown coloured [Fe(III)-HMBAHBH] water soluble complex. The maximum absorbance was observed at 390 nm at pH 3.0. Beer’s law is obeyed in the concentration range of 0.14 – 4.19 μg mL-1. The molar sensitivity and Sandell’s sensitivity of the coloured complex was found to be 1.87 x 104 L mol-1 cm-1 and 2.9x10-3 μg cm-2. The stoichiometric studies of the Fe (III) complex is determined as 1:2 with formation of stability constant 1.02x1011. The analytical results of the complex is subjected for treatment by statistical methods and statistical data is obtained as 0.3580 (slope), -0.0091(Y-intercept), 0.012(relative standard deviation) and 0.9997 correlation coefficient. The interference effect of various diverse ions has been studied. All the proposed methods are successively employed in the analysis of various surface soil samples, biological, food materials and pharmaceutical samples for the determination of Iron (III) content.

Keywords: Iron (III), HMBAHBH, Triton X-100, surface soils, biological, food materials and pharmaceutical samples, UV-Visible spectrophotometer.