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Abstract

PHYTOCHEMICAL INVESTIGATION AND EVALUATION OF ANTIMICROBIAL AND ANTITUBERCULAR ACTIVITY OF KUNSTLERIA KERALENSIS

Kumar M.D*, Sathishkumar shetty A, Sathyanarayan N.D, Vijay kumar M.L, Kuppast I.J and Vasanthkumar Pai K

ABSTRACT

The hexane, chloroform and methanolic extract of bark and leaf of the plant“Kunstleria keralensis” belonging to the family Fabaceae were screened for antimicrobial and antitubercular activity. The antimicrobial activity was carried against five target bacteria and five target fungi by disc diffusion method. The hexane extract (HB), chloroform extract (CB) of bark and hexane extract of leaf (HL) showed significant antibacterial activity against Klebsiella pneumoniae, Escherichia coli and Proteus vulgaris. The further evaluation of test samples by minimum inhibitory concentration (MIC) showed that test sample HB had significant activity by inhibiting the target bacteria K. pneumoniae at 50μg/ml, E. coli and P. vulgaris at a concentration of 100μg/ml. The test samples CB and HL inhibited the target organisms at 100μg/ml and 200μg/ml on different target organisms. The test sample HL exhibited significant antitubercular activity by inhibiting the growth of Mycobacterium tuberculae at 25μg/ml. The other test samples exhibited antitubercular activity at 50μg/ml. However, none of the test samples exhibited antifungal activity.

Keywords: Antimicrobial activity, antitubercular, Kunstleria keralensis, solvent extraction, human pathogens.


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