Abstract

FOMULATION AND EVALUATION OF GELATIN NANOPARTICLES FOR PULMONARY DRUG DELIVERY

Nitan Bharti*, S.L.Harikumar, Shishu, Abhishek Buddiraja

ABSTRACT

Pulmonary drug delivery is a non-invasive, non-systemic delivery approach for both local and systemic drugs and a method to directly target disorders of lung. The gelatin nanoparticles were prepared by double desolvation method using different concentration of gelatin as biodegradable polymer for lungs. The prepared gelatin nanoparticles were evaluated for entrapment efficiency, particle size, polydispersity index, zeta potential, transmission electron microscopy, in-vitro drug release, stability study and in-vivo study. The entrapment efficiency of all gelatin nanoparticles formulations were found to be in the range of 46.16 % - 58.60 %. The particle size of gelatin nanoparticle formulations(GNps1, GNps2, GNps3, GNps4) were found to be 179.9 nm, 198.4 nm, 252.4 nm and 1545 nm,respectively. The gelatin nanoparticle formulation GNps3 was selected best formulation depending upon the particle size less then 500nm and higher entrapment efficiency as compared to GNps1 and GNps2. The zeta potential of gelatin nanoparticles found to exhibit stability due to positive charge on the surface. The transmission electron microscopy indicated the spherical surface of the gelatin nanoparticles. The in vitro release was found to follow higuchi plot as compared to zero order plot, first order plot and krosmeyer peppas plot. Stability studies showed that gelatin nanoparticle formulation GNps3 was stable when tested for particle size, entrapment efficiency and in vitro drug release at refrigerated condition (5º±3ºC), at room temperature (25º±2ºC/65%±5% RH) and at accelerated condition (40º±2ºC/75%±5% RH) according to ICH guidelines for 6 months(180 days). In-vivo study using rat model indicated the localization gelatin nanoparticles in the lungs of rat.

Keywords: Gelatin nanoparticles, pulmonary drug delivery, terbutaline sulfate, particle size, entrapment efficiency, stability.