Abstract

COMPUTATIONAL APPROACHES TO CHARACTERIZE THE NOVEL BIOSYNTHETIC PATHWAYS OF ANTIBIOTIC PRODUCING ACTINOMYCETES

K.Aditya*, B.K.Manjunath, D. Jagadesh Kumar, and Kusum Paul

ABSTRACT

The present work aims at identification and taxonomical characterization of antibiotic producing microorganisms, by comparing the nucleotide sequences of isolates and referred strains. Present analysis attempts to throw light on functional characters of the unknown isolates using in-silico techniques. The 20 closest species to our unidentified sequence with identity between 97% - 99% were selected via BLAST, and a phylogenetic tree was constructed. Sequences belonging to Streptomyces Spp. like S.atratus, S.bacillaris, S.kanamyceticus etc., were selected for detailed molecular characterization and pathway analysis, in order to discover the ability of these isolates towards producing novel therapeutic substances. Our results show that, these isolates have the aptitude to produce antibiotics for typhoid like ailments, and few of them, also exhibit the promise to behave like anti-tumor agents. Supplementary analysis for biosynthesis pathway of antibiotics was conducted to understand the activity of these antibiotics. Predicted functional analyses of the three strains demonstrate the capability to produce antibiotics like Rifamycin, Streptomycin, and Kanamycin.

Keywords: Actinomycetes, Streptomyces, secondary metabolites, phylogenetic analysis.