Abstract

BACTERIOLOGICAL PROFILE, ANTIBIOGRAM OF BURN WOUND ISOLATES AND DETECTION OF MRSA AND ESBL PRODUCTION AT TERTIARY CARE HOSPITAL, HYDERABAD

S. Rajeshwar Rao, L. Jaya Lakshmi, S.Pavani, Vijendra Kawle3, S. Jaya Prakash

ABSTRACT

Introduction: Infection is an important cause of morbidity and mortality in burn patients. Identification of bacterial isolates from specimens of burns patients and study of their drug susceptibility pattern helps in determining empirical antimicrobial therapy to burns patients. Aim: To identify the common aerobic bacterial isolates and study their antimicrobial susceptibility in specimens collected from burn wounds including methicillin resistance in Staphylococci and production of extended spectrum beta lactamases (ESBL) in Enterobacteriaceae isolates. Material & Methods: A total of 1600 Specimens from burn wounds received at Microbiology laboratory of the tertiary care hospital, Hyderabad from July 2012 to December 2013 were included in the study. The specimens were processed according to standard laboratory protocols, isolates were identified by conventional biochemical methods and antimicrobial susceptibility was performed by Kirby-Bauer disc diffusion method. Results: Among 1600 specimens, 1043 showed single bacterial growth and 75 showed mixed bacterial isolates thus total bacterial isolates were 1193. The commonest isolate was Staphylococcus aureus (42%) followed by Klebsiella species (31%). Other isolates include Pseudomonas aeruginosa (7.8%), Coagulase Negative Staphylococci (6.96%), Escherichia coli (5.11%), Citrobacter species (3.6%), Proteus species (3.2%) and Acinetobacter species (0.33%). Among 501Staphylococcus aureus isolates 32.7% were Methicillin Resistant Staphylococcus Aureus (MRSA) and among 512 isolates of Enterobacteriaceae 30.9% were ESBL producers. Conclusion: Staphylococcus aureus and Klebsiella species were the common organisms causing burn wound infection in the present study. The susceptibility patterns in thepresent study indicate fluoroquinolones were effective against Gram negative bacilli after Imipenem and against Gram positive bacteria after Vancomycin and thus can be used for empirical therapy.

Keywords: burns wound, aerobic bacterial isolates, Staphylococcus aureus, Klebsiella species, MRSA, ESBL.