Abstract

DEVELOPMENT AND CHARACTERIZATION OF ATENOLOL AND INDAPAMIDE IN THEIR COMBINED DOSAGE FORM USING DIFFERENT POLYMERS

Ashutosh Badola*, Guno Sindhu Chakraborthy

ABSTRACT

Dosage containing drugs Atenolol and Indapamide are used to treat the symptoms of Hypertension, a condition technically known as increase in Blood pressure. Several chromatographic methods have been reported for simultaneous estimation of these drugs and individual drug. In the present study a simple, sensitive, accurate and effective Reverse Phase High-Performance liquid chromatographic (RP- HPLC) method was developed for the determination of Atenolol and Indapamide in combined dosage form. The analysis was resolved by using different chromatographic conditions by altering mobile phase and flow rate mechanism and HPLC system consisting of Inertsil ODS (C18, 150*4.6mm), 5nm column at a wavelength of 242 nm. The retention time for the drugs was found for Atenolol 2.2 min and for Indapamide 4.3 min respectively. As in simultaneous estimation of these drugs it was found that a confined release can be formulated.The effect of different polymers on release of the sustained release tablets of the Atenolol and indapamide was evaluated. The IR spectrum study revealed that there is no disturbance in the principal peaks of pure drugs. This confirms the integrity of pure drugs and no incompatibility of them with excipients and formulationshows zero order release rate, which is sign of sustained release.

Keywords: HPLC, Atenolol, Indapamide, Zero order, simultaneous estimation, sustained release