Abstract

STUDY OF EFFECT OF TEMPERATURE ON AMYLASE PRODUCTION BY SOIL MYCOTIC FLORA OF JABALPUR REGION

Sonal Sareen Pathak, Suneel Kumar, R. C. Rajak, Sardul Singh Sandhu*

ABSTRACT

The effect of temperature on enzyme activity shows that enzyme from different fungal isolates have their optimum temperature. The nutritional and cultural conditions are required for the optimum growth and production of amylase from the mycotic flora. The present study deals with the isolation of amylolytic fungi from soil samples. The fungal isolates showing amlolytic activity are used to extract the crude amylase to study the effect of temperature for optimum production of extracellular amylase. In the present research work, Aspergillus niger, Aspergillus flavus, Rhizopus sp. and Fusarium sp. were isolated from the soil sample. A part from these isolates, Absidia species, Alternaria alternata, Curvularia lunata and Pythium species were also reported in the samples. Among them, the maximum amylolytic activity was showed by Aspergillus niger and Aspergillus flavus. The enzyme activity was observed for the isolates at different temperature range of 28°C, 42°C and 60°C and it was inferred that the activity varied for different isolate at different temperature. The production of amylase is influenced by temperature which is related to the growth of organism.

Keywords: Amylase, temperature, optimum, extracellular, amylolytic.