Abstract

STUDY OF ANTIOXIDANT ACTIVITY OF ETHYL ACETATE FRACTION OF HIBISCUS VITIFOLIUS STEM BARK AND ITS ISOLATE.

I.Carolin Nimila, K.G.Lalitha*

ABSTRACT

Objective: Hibiscus vitifolius Linn. (Family- Malvaceae) commonly known as manjaltutthi (in Tamil) is an annual or perennial herbaceous plant, traditionally used in treatment of jaundice. Since the antioxidant property has significant role in many diseases, the present study was Undertaken with an aim to isolate and characterize the phytoconstituent and to evaluate the in-vitro antioxidant activity of H. vitifoliusstem bark. Methods: On column chromatography phytoconstituent was isolated and characterized by spectral studies. Standard in-vitro antioxidant assays such as reducing power, super oxide scavenging, nitric oxide scavenging and lipid peroxidation were performed. Results: From the results of spectral studies, the isolated compound was characterized as kaempferol. The H. vitifolius stem
bark extracts exhibit potent antioxidant effect compared to the standards used in the study. Conclusion: The present study demonstrates the potentiality of H.vitifolius stem bark extracts and isolated compound kaempferol as an antioxidant. Kaempferol was found to be one of the important component responsible for antioxidant activity of the H. vitifolius stem bark.

Keywords: Antioxidant, Hibiscus vitifolius, Kaempferol.