Abstract

PROTECTIVE EFFECT OF ETHANOLIC ROOTS EXTRACT OF PSEUDOCEDRELA KOTSCHYI (PK) ON SOME HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS IN ALLOXAN-INDUCED DIABETIC RATS

ABSTRACT

This study investigated the effect of ethanolic roots extract of Pseudocecrela kotschyi on some hematological and biochemical parameters in alloxan-induced diabetic rats. Twenty- Five (25) wistar rats were randomly divided into five (5) experimental groups: control, diabetic, standard drug (glibenclamide) and Pseudocedrela kotschyi treated diabetic groups. The animals in four out of five groups were fasted for 18 h and were made diabetic by injecting with a single dose of alloxan (ALX) 150 mg kg-1. At the end of experimented period of 21 days (3 weeks), rats were sacrificed and blood samples were taken for the determination of glucose levels, red blood cell (RBC) count, white blood cell (WBC) count, packed cell volume (PCV), hemoglobin (Hb) concentration, and platelet count. Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), urea and creatinine levels significantly increased (p<0.05) in diabetic rats as compared to control. By contrast, significant reduction (p<0.05) of the erythrocyte and leukocytes counts and pack cell volume (PCV) levels in diabetic group were also noticed in diabetic rats as compared to control. Treatment of diabetic rats with this extract caused a significant decrease (p<0.05) in blood glucose levels, urea and creatinine, whereas ALT and AST slightly lowered. In addition, in diabetic treated groups, the erythrocyte and leukocyte counts, Hb and PCV values significantly increased (p<0.05), whereas the leukocyte count slightly increased. We concluded that oral P.kotschyi treatment might decrease the diabetic-induced disturbances of some hematological and biochemical parameters in alloxan-induced diabetic rats.

Keywords: Antioxidants, blood cells, hyperglycemia, rats, alloxan, Pseudocedrela kotschyi.