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Abstract

DIRECT AND DERIVATIVE SPECTROPHOTOMETRIC DETERMINATION OF COPPER (II) USING A SENSITIVE AND SELECTIVE CHROMOGENIC ORGANIC REAGENT 2-HYDROXY 3,5-DIMETHOXY BENZALDEHYDE THIOSEMICARBAZONE (HDMBTSC)

M.Hymavathi, N.Devanna and  C.Viswanatha

ABSTRACT

2-hydroxy 3,5-dimethoxy benzaldehyde thiosemicarbazone (HDMBTSC) is used as a novel chromogenic organic reagent for the determination of Copper (II) with specterophotometry.The chromogenic reagent 2-hydroxy 3,5-dimethoxy benzaldehyde thiosemicarbazone (HDMBTSC) form light yellowish green coloured complexe with Copper(II). The colour complexe shows maximum absorption at max 397nm.The beer's law validity range is 0.1588 to 1.5886 (µg/ml) and optimum concentration range is 0.3177 to 1.4297 (µg/ml). The molar absorptivity and sandells sensitivity 3.06x104 L.mol-1.cm-1 and 0.00207 g/cm2 respectively. The Copper(II) forms M:L(I:I) colour complexe with HDMBTSC and stability constant of the complex was found to be. 1.72x 106. The first and second derivative amplitude was measured at 465 nm and 483 nm. This method was applied for the determination of Copper(II) in bear and wine samples with good results.

Keywords: Novel chromogenic organic reagent, derivative spectrophotometry, Cu (II), bear and wine samples.


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