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Abstract

UV SPECTROPHOTOMETRIC ESTIMATION OF ACYCLOVIR IN BULK AND TABLET DOSAGE FORM USING AREA UNDER CURVE METHOD

Bhagyashri P.Pawaskar*, SugandhaV. Mulgund

ABSTRACT

This paper describes development and validation of UV spectrophotometric method for the estimation of Acyclovir in tablet formulation using area under curve method. The standard and sample solutions of Acyclovir were prepared in distilled water. The area under curve in between 241to 251 nm was measured. The method followed linearity in the range of 2-12μg/ml with correlation coefficient value of 0.999. This method was validated for various parameters according to ICH guideline Q2 (R1). Satisfactory values of Percent relative standard deviation for the intra-day and inter-day precision indicated that method was precise. The mean percentage recovery of analyte was found to be 99.41%. LOD and LOQ were calculated as 0.3134μg/ml and 0.9515μg/ml, respectively.

Keywords: Acyclovir, UV spectrophotometry, Area under curve, Validation.


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