Abstract

DEVELOPMENT AND CHARACTERIZATION OF NOVEL ENZYMES

Amol S.Rakte*, Basavaraj K.Nanjwade

ABSTRACT

Aim of the present research was to formulate and evaluate a multienzyme (Bromelain, Papain, Bacterial Protease, Rutin, and Vitamin C) effervescent tablet with sufficient mechanical integrity, good palatability, robust formulation, good stability, inexpensive and shop floor friendly product produced in normal thermo-hygrometric conditions. In the preformulation study, compatibility evaluation was performed which implies that drug and excipients were compatible with each other. The formulations of tablets were done by using wet granulation, dry granulation and direct compression method. Total ten trials for each method was prepared and evaluated for hardness, friability, effervescent time, weight variation, and pH. Finally one trial from each method was kept for stability TF-6 (direct compression) TF-7(dry granulation) and TF-8(wet granulation). From that techniques stability of tablets with wet granulation method was found to be satisfactory as compared to dry granulation and direct compression. Formulation (TF-8) with wet granulation containing citric acid (12.46%), tartaric acid (24.92%), sodium bicarbonate (37.40%), sodium carbonate (5.95%), povidone (2.99%) consider the final formulation.

Keywords: Effervescence, Enzymes, Food chemical codex (FCC), Carbon dioxide content.