Abstract

UV SPECTROPHOTOMETRIC ESTIMATION OF DILTIAZEM HYDROCHLORIDE IN BULK AND TABLET DOSAGE FORM USING AREA UNDER CURVE METHOD

Ashwini Nikhade*, Sugandha Mulgund

ABSTRACT

This paper describes development and validation of UV spectrophotometric method for the estimation of Diltiazem hydrochloride in tablet formulation using area under curve method. The standard and sample solutions of Diltiazem hydrochloride were prepared in distilled water. The area under curve in between 226 to 246 nm was measured. The method followed linearity in the range of 6-16 μg/ml with correlation coefficient value of 0.999. This method was validated for various parameters according to ICH guideline Q2 (R1). Satisfactory values of Percent relative standard deviation for the intraday and inter-day precision indicated that method was precise. The mean percentage recovery studies was found to be 105.69 %. LOD and LOQ were calculated as 0.3197 μg/ml and 0.9689 μg/ml, respectively

Keywords: Diltiazem hydrochloride, UV spectrophotometry, Area under curve, Validation