Abstract

IN VITRO ANTI PROLIFERATIVE, ANTI OXIDANT AND PHYTOCHEMICAL SCREENING OF A SIDDHA DRUG CHITTIRAMOOLA KULIGAI (CMK)

Ravichandran.M*, Mubarak.H

ABSTRACT

Siddha medicines are time tested formulations and having abundant anticancerous drugs cited in many classical references. Chittiramoola Kuligai (CMK) is a known drug and it is indicated for Chronic ulcers, Cancerous conditions of urogenital regions, chest regions and eight types of Gunmams (acute and chronic abdomen). The CMK is tested against HeLa cell lines which represents cervical cancer. The cell viability is applied through MTT assay technique. The different concentrations of the CMK (10 μg/ml, 50 μg/ml, 100 μg/ml) were tested. The IC 50 value was 83 μg/ml. The anti oxidant activity via different methodologies Nitric oxide and DPPH assay method were applied. The CMK is showed good anti oxidant activity by lesser concentrations. The phyto chemical analysis revealed the presence of Alkaloid, Flavanoids and Triterpinoids. These phytochemicals components may be helpful in anticancerous mechanisms. Finally the drug can be taken as choice of drug to treat cervical cancer patients and the clinical evidences must be documented.

Keywords: Siddha, Chittiramoola Kuligai, MTT assay.