Abstract

COMPARATIVE PHYTOCHEMICAL QUANTIFICATION IN GREEN AND PURPLE VARIETIES OF BASELLA ALBA FOR PREVENTIVE MANAGEMENT OF NUTRITIONAL DEFICIENCY AND STRESS RELATED DISEASES

B.Thirupathi, S. Gangadhar Rao*

ABSTRACT

Antioxidant secondary metabolites, antioxidant enzyme activities and antioxidant radical scavenging assays were done in green and purple varieties of nutritionally important Basella alba Content of phenols ,flavonoids, flavonols, proanthocynidins did not differ much between green and purple varieties, however anthocyanins was recorded maximum in purple variety 1.655 as compared to green variety(0.285mg/g dr.wt). Total carotenoid and βcarotene showed high concentration 2.48 and 0.229 mg/g. Fr. wt in green compared to 1.55 and 0.44 mg/g fr.wt in purple variety Enzymes peroxidase and glutathione reductase showed maximum activity in green variety with 1.25 and 0.194 units as compared to 1.066 and 0.1327 units in purple variety respectively. Catalase and polyphenol oxidase showed maximum activity in purple variety 0.73 and 0.532 units as compared to 0.56 and 0.357 units green variety respectively. Radical scavenging activity assays i.e. FRAP, ABTS and DPPH inhibition % has showed maximum24.56, 37.5, 66.46 % activity in purple variety respectively when compared to 18.07, 14.58 and 59.75 % activity in green variety respectively.

Keywords: Antioxidants, secondary metabolites, Antioxidant assays, phytochemicals, Basella alba varieties.