Abstract

EFFICIENT METHOD FOR IN VITRO PLANT REGENERATION FROM COTYLEDONARY NODE EXPLANTS OF CITRULLUS COLOCYNTHIS (LINN.) SCHRAD

Mahesh Chand Meena*, Rishi Kesh Meena, Vijay Prakash Meenaand Vidya Patni

ABSTRACT

Citrullus colycynthis (Linn.) Schrad. is an important medicinal herb of belonging to family Cucurbitaceae. The present study is focused on induction of multiple shoots from cotyledonary node explants of C. colocynthis. Cotyledonary node explants were excised from 7 day old seedlings and were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and Kn (0.5- 5.0mg/l) and BAP in combination with (0.5-3.0 mg/l) auxins for shoot bud initiation. The maximum number of shoot bud regeneration (35.4 ± 0.220 shoots /explant) was observed on MS-medium supplemented with BAP (1.5 mg/l) and NAA (1.5 mg/l).

Keywords: Cotyledonary node, In vitro, shoot bud regeneration, Citrullus colocynthis.