Abstract

POMEGRANATE JUICE AFFECTS ON PHARMACOKINETIC PARAMETERS OF METRONIDAZOLE BY USING HPLC-MS

Haifa T. Abu Tbeekh, Wael A. Abu Dayyih*, Eyad M. Mallah, Nidal A. Qinna,Riad M. Awad, Tawfiq A. Arafat

ABSTRACT

Objective: To quantify Metronidazole in rat serum by using HPLC/MS and to study the effect of pomegranate fresh juice on the Metronidazole pharmacokinetics Methods: The serum samples were prepared by using protein precipitation method with Clindamycin in methanol solution. A gradient mobile phase system and ACE 5 C18 column were used for the analysis. Rats (n=4) were treated with a single ordinary dose (5ml/kg) of freshly squeezed pomegranate juice 30 min before orally administered-Metronidazole while other groups of rats (n=4) were treated with a multiple dose of the juice twice daily for two days versus DW pretreated rats (n=2). The study protocol was approved by the ethical committee of Graduate Studies at Faculty of Pharmacy and Medical Sciences of University of Petra/Amman-Jordan (March, 2012). Key findings: The analytical method was linear with acceptable recovery, precision and accuracy. The results show that there was a significant increase in the Metronidazole pharmacokinetic parameters (Cmax and AUC, P<0.05) after pretreatment with multiple dose of pomegranate juice. Conclusions: Pomegranate juice with a multiple dose pre-treatment could be a good intestinal but not hepatic enzymes inhibitor for Metronidazole metabolism.

Keywords: Pomegranate, Metronidazole, Pharmacokinetic, HPLC/MS.