Abstract

EVALUATION OF DIURETIC ATIVITY OF TECTONA GRANDIS IN RATS

¹S. G. Phalphale*, ¹Ashish Gawai, ¹K.R. Biyani, ²R.V. Shete, ²K.J. Kore, ³S.R.Chaudhari, ¹Samadhan Magar.

¹Department of Pharmacology, Anuradha College of Pharmacy, Chikhli, M.S, India.
²Department of Pharmacology, Rajgad Dnyanpeeth’s College of Pharmacy, Bhor. M.S, India.
³Department of Pharmacology, L.M. C. College of Pharmacy, Faijpur. M.S, India.

ABSTRACT

Diuretic is any substance which increases the urine and solute excretion. The pharmacological modulation of volume is targeted at reducing volume excess; this is effective treatment for heart failure, as well as cirrhosis and the nephritic syndrome. Traditionally, Tectona grandis is used as diuretics. The preliminary phytochemical screening of the aqueous extract of Tectona grandis revealed the presence of phenolic compounds, carbohydrates, saponnins, tannins and flavonoids. So aqueous extract of Tectona grandis was selected for scientific base of its diuretic evaluation. LD50 value of aqueous extract of Tectona grandis was above 2000 mg/kg body weight. Adult male Wistar rats 150-200 g was used for diuretic studies using aqueous extract of Tectona grandis administered orally. A five groups consisted of 6 rats were placed in a metabolic cages and treated with Hydrochlorothiazde as a standard and three different doses (100, 200 & 400 mg/kg) of aqueous extract of Tectona grandis .The urine excreted over a period was measured at every 2, 4, 6, 8 and 24 hrs. for single rat was collected and measured. Urinary Na+, K+ and Cl- contents for each group after 24 hours were analyzed by Auto analyzer. The present study has indicated that the aqueous extract of Tectona grandis in three doses showed diuresis at different time interval and there was significant increase in urinary Na+, and Cl- excretion. Results were calculated by Dunnet,s test.

Keywords: Tectona grandis, diuretic, urinary excretion, Na+, and Cl-