Abstract

PHARMACOLOGICAL PROPERTIES OF CORM ETHANOL EXTRACT OF MUSA PARADISIACA (L.) CV. PUTTABALE

Venkatesh, Krishna Venkatarangaiah*, Pradeepa Krishnappa, Santosh KumarShimoga Rajanna, Mir Haris, Vijay Keriyappa

ABSTRACT

To evaluate the anticonvulsant, CNS depressant and antinociceptive properties of corm extract of M. paradisiaca cv. Puttabale on in vivo models. The quantification and identification of phenolics and flavonoids were determined in crude extract using HPLC-UV analysis. The LD50 was determined and extracts were used at doses of 100, 200 and 300 mg/kg for the studies. The anticonvulsant activity was evaluated using Maximum electroshock (MES), Pentylenetetrazole (PTZ) and locomotor test. For the evaluation of CNS depressant properties, forced swim test, muscle co-ordination test and antinociceptive action of abdominal writhing, tail-flick test, thermalinduced pain models were used in experiments. The extract depicted total phenolics (628.6 μg/mg) and flavonoids (321.6 μg/mg) and caused a significant reduction of MES (4.33 ± 0.49), PTZ (168.83 ± 1.49) and locomotor test (129.33 ± 1.43). The extract also reduced the reaction time of forced swim test (59.50 ± 1.34) and muscle coordination test (89.32%). As well, extract reduced significantly nociception induced by acetic acid (65%), tail-flick test (90 min) and hot plate (180 min). The results suggest that the corm extract of M. paradisiaca cv. Puttabale possess anticonvulsant, CNS depressant and antinociceptive properties which may be attributable to the presence of phenolics and flavonoids in the plant.

Keywords: Musa paradisiaca cv. Puttabale, corm ethanol extract, Maximum electroshock, Pentylenetetrazole, Forced swim test, Muscle co-ordination test, acetic acid-induced writhing.