OPTIMIZATION OF EXTRACTION CONDITIONS AND ANTIMICROBIAL POTENTIAL OF A BIOACTIVE METABOLITE, CORDYCEPIN FROM CORDYCEPS MILITARIS 3936
Hardeep S. Tuli, S. S. Sandhu*, Dharambir Kashyap and Anil K. Sharma
ABSTRACT
Cordycepin, a nucleoside derivative, is an active component of
parasitic fungus, Cordyceps militaris which has been known for many
pharmacological activities. The present study was carried out to
develop an extraction strategy for cordycepin and to evaluate its
antimicrobial potential. Solvent- solvent extraction method was used to
extract cordycepin from liquid culture of C. militaris. Crude
concentrated extract of fermented broth was sequentially partitioned
with hexane, chloroform and n-butanol. Further we assessed the effect
of solvent-solvent ratio, extraction time and temperature on cordycepin
extraction Antimicrobial properties of various Cordyceps militris
derived fractions such as hexane; chloroform and n-butanol were investigated against human
pathogenic microorganisms using the disc diffusion. Maximum yield of cordycepin was
achieved at a solvent ratio of 1:2 (v/v) with extraction time of 90 minutes at 40°C. Final
purity of the cordycepin was recorded up to 95% based on high performance liquid
chromatography. Butanolic fractions, demonstrated the highest antimicrobial activity, due to
the presence of cordycepin as an active component. The minimum inhibition concentration
(MIC) of cordycepin was found to be 25-40 μg/disc for all the microorganisms tested. Hence
Cordyceps militaris derived cordycepin could potentially be used in the treatment of
infectious diseases caused by pathogenic microorganisms.
Keywords: Cordyceps militaris, solvent-solvent extraction, antimicrobial, purification, cordycepin.
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