STABILITY INDICATING RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PARACETAMOL AND PAMABROM IN BULK AND COMBINED PHARMACEUTICAL DOSAGE FORM
Dhruvi Shah*, Bhagirath Patel, Ankita Bhavsar
ABSTRACT
A simple stability indicating high-performance liquid chromatographic
method was developed and validated for simultaneous estimation of
Paracetamol and Pamabrom in bulk and combined pharmaceutical
dosage form. Reversed-phase chromatography was performed on
Shimadzu Model SPD-20AT, using a mixture of Phosphate buffer:
Methanol (65:35v/v) as a mobile phase with a flow rate of 1.0 ml/min.
Detection wavelength was selected 270nm. The retention time for
Paracetamol and Pamabrom was found to be 4.04 min and 7.36 min
respectively. The linearity of developed method was achieved in the
range of 32.5-97.5μg/ml for Paracetamol and 2.5-7.5μg/ml for
Pamabrom. The Paracetamol and Pamabrom were subjected to stress conditions including
acidic, alkaline, oxidation, thermal and sunlight degradation. The method was validated as
per ICH guidelines.
Keywords: RP-HPLC, Stability indicating method, Paracetamol (PCM), Pamabrom (PAM).
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