Abstract

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR ESTIMATION OF CANDESARTAN FROM TABLET DOSAGE FORM

Syeda Kulsum*, G Vidya Sagar, K. Nagalakshmi, R. Snehalatha

ABSTRACT

A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of candesartan in both pure and pharmaceutical dosage forms. An Inertsil ODS-3V C-18, 5 um column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing buffer (0.5% tri ethyl amine)and methanol (50:50%v/v) and adjust the pH to 4.5 by using glacial acetic acid. The flow rate was 1.0 ml / min and the effluents were monitored at 210nm. The retention time was 8.057min. The linearity was in the range of 50-160 g / ml. This method was validated for linearity, precision, limit of detection, limit of quantitation and accuracy. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.

Keywords: RP-HPLC, Candesartan, Validation,mobile phase,inertsil column.