Abstract

STUDYING OF ANTIBACTERIAL EFFECT FOR WATERY AND ALCOHOLIC EXTRACTS OF PIPER NIGRUM L FOR SOME PATHOGENS IN VITRO AND IN VIVO

Afrah J. Abd, *Noor S. Aboud, Haifaa J. Hassan

ABSTRACT

This study was designed to evaluate the effects of the watery and alcoholic extracts from leaves of pepper nigrum L in vitro on ten pathogenic factors in addition to studying the antibacterial effect of watery leaves extract toward proteus vulgaris in vivo. Results showed that the watery and alcoholic extracts from leaves pepper nigrum L have antibacterial activity against both Gram positive (staphylococcus aureous, streptococcus pneumonia) and Gram negative bacteria (klebsilla pneumonia ,proteus vulgaris, Escherichia coli, vibrio cholera and Salmonella paratyphi) in addition to candidia albicanes. However watery extract of pepper nigrum was more potent than alcoholic extract against pathogenic factor. According to results proteus vulgaris was the more sensitive pathogenic factor toward pepper nigrum L, thus watery extract was chosen to injected intraperitonialy in the laboratory rabbits, which cause morphological and pathological degenerative lesion for hepatic, renal tissues in addition to changes in hepatic function indicators, cardiac and renal profile tests which include total serum bilirubin, aspartame aminotransferase, alanine aminotransferase, alkaline phasphatase, gama-glutamle transaminase for hepatic indicator, while urea, creatinine, uric acid, potassium and creatinine kinase for renal tests while cardiac tissue and indicators that includes trponine, myoglobin and CK –MP, were did not affected any mater. Watery extract of pepper nigrum L cause significant improvement (p≤ 0.01) in the value both renal and hepatic tests for injected rabbits. histopathological studies confirm these results which include regeneration of degenerative lesion portal area and moderate congestion renal medulla and cortex tissue.

Keywords: pepper nigrum L, antibacterial, in vitro, in vivo, Proteus vulgaris.