Abstract

ANTIBACTERIAL, ANTIFUNGAL AND PRELIMINARY PHYTOCHEMICAL INVESTIGATION OF LYCOPERDON UMBRINUM

C. Ashok*, K. K. Jayashree, J. Suma, K. Pushpa M. S. Shruthi, and N. Raja

ABSTRACT

The aim of present study is to investigate the antibacterial, antifungal and preliminary phytochemical activity of crude extracts of Lycoperdon umbrinum in different solvents (petroleum ether, chloroform and methanol) as a possible source for new antimicrobial substances against some pathogens. We evaluated the antibacterial and antifungal activity of crude extracts of Lycoperdon umbrinum against some human as well as plant pathogens viz. nine bacterial strains were used X. campestris, P. syringae, A. tumefaciens, E. coli, K. pneumoniae, P. aeuroginosa and S. typhi, gram negative. S. aureus and S. pneumoniae, gram positive and nineteen fungal strains were used, A. alternate, A. flavus, A. solani, A. tomentosa, C. capsici, C. dematium, C. lindemuthianum, F. oxysporum and F. solani, plant pathogens. M. gypseum, T. equinum, T. kanei, C. albicans, C. indicum, C. krusei, C. merdarium, C. keratinophilum, E. floccosum, T. rubrum, human pathogens. The dried and powdered were successively by cold extraction. The antimicrobial assay was done by agar-well diffusion method. All the three extract of Lycoperdon umbrinum show highest activity against all the bacterial strains (gram negative and positive pathogens), whereas, moderate activity shows against some fungal strains (human and plant pathogens) such as C. dematium (4-8 mm), T. kanei (5.3-12 mm), C. albicans (6.3-14 mm), C. krusei (7.3-13 mm) and T. rubrum (5.3-13.2 mm), to varying degrees by all the extracts. Tetracycline, Clotrimazole, Fleuconzole, Mancozeb and Captan were used as the standard antibiotics against bacteria and fungi. The extracts of Lycoperdon umbrinum also significantly inhibited the bacterial and fungal growth.

Keywords: Lycoperdon umbrinum, Antimicrobial process, Polar solvents, Microorganisms, Wild mushrooms, Western Ghats.