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Abstract

DETECTION OF HUMAN BOCAVIRUS IN IRAQI PEDINTRIC PATIENTS SUFFERING FROM RESPIRATORY INFECTION USEING ELISA AND PCR METHODS

Dr. Zaid Taha Yaseen, Dr. Mohammed Khalifa Khudair* and Asmaa Saleh Naseef

ABSTRACT

This study was conducted in Al Muqdadiya district in Diyala province. A total 180 samples were collected from pediatric patients who were in Al-Zahra Hospital for gynecology and pediatrics.Were Included both sexes and ages ranging from (3 months to 10 years) whose suffering from respiratory infections. The study samples were divided into two groups. First group consisting of (80) blood samples for patients and (10) samples as control group for healthy people, the second group (80) Sample swabs from the pharynx and control group composed of (10) healthy people. The results showed the prevalence of (HBoV) using PCR technique was )38.3%( with significant difference )P<0.05( when compared with control group, age group (3 months -1years) recorded the highest rate of infection (43.5%(, followed by (5-7) years )42.9%(, and from (2-4) years )33.3%) while the lowest percentage in the age group (8-10 years) )28.5%) with non a significant difference between age groups ) P>0.05(.The percentage of infection among males was (40.0%) and females (37.1%) with no significant difference (P>0.05). The study demonstrated an increase the antibodies to HBoV in patients compared to healthy, The IgG ratio was (35.0%) with a significant difference when compared with control group )P <0.05(. A high rate of IgG was recorded in age group (3months -1 years) (51.3%), followed by the category of (5-7) years consisted (28.6%( and (2-4) years consisted )22.2%(, while in age group (8-10) years was not detected, with a significant difference between age groups )P<0.05(. The results illustrated an increased in the percentage of infection among males was )37.8%( while in females was )31.4%( with no significant difference (P>0.05).The prevalence of IgM antibody was )8.8%( and a significant difference when compared with control group(P<0.05).

Keywords: Human Bocavirus (HBoV), respiratory tract infections, PCR, Elisa.


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