Abstract

ANTIMICROBIAL ACTIVITY OF DIFFERENT EXTRACTS FROM LEAVES OF A SPECIFIC MEDICINAL PLANT(Senna obtusifolia)

*Mohammed Yousuf, Md. Rafiqul Islam Rupam, Sushanta Halder, Md. Imran Nur Manik and Prof. Dr. Md. Harun Ar Rashid

ABSTRACT

This study was carried out to evaluate antimicrobial activity of the crude methanolic extract and its different partitionates i.e. petroleum ether (PEP), dichloromethane (DCM), ethyl acetate (EtOAc) and aqueous (AQP) soluble fractions of leaves of S. obtusifolia against some pathogenic bacteria. The powdered leaf of S. obtusifolia was extracted with methanol. The ethyl acetate extract exhibited the highest inhibition against microbial growth having zone of inhibition 8 - 20 mm. The maximum zone of inhibition produced by EtOAc was found to be 20 mm against Sh. dysenteriae followed by 12 mm against Escherichia coli. This partitionate also showed antibacterial activity against Bacillus cereus, B. subtilis and B. megaterium (having zone of inhibition of 11-8 mm) and Staphylococcus aureus and Sarcinalutea (having zone of inhibition of 9 mm and 11 mm). The extractive also showed antifungal activity against Sacharomycescerevacae (6 mm), Candida albicans (12mm) and Aspergillusniger (7mm). The dichloromethane soluble fraction (AQP) also exhibited mild to moderate inhibition against microbial growth having zone of inhibition 4 - 12 mm. The maximum zone of inhibition produced by DCNSF was found to be 12 mm against Sarcinalutea.

Keywords: Extract, Extraction, Zone of inhibition, Antimicrobial activities, Partitionate, Fabaceae.