Photo Gallery

Login

Search

News & Updation

  • WJPPS APRIL ISSUE PUBLISHED
  • APRIL 2019 Issue has been successfully launched on 1 April 2019

  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from 6.647 to 7.421 due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  

Abstract

ANALYTICAL RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF NEVIRAPINE IN API AND PHARMACEUTICAL DOSAGE FORM

Bonagani Naresh*, Manda Rakesh, D. Pallavi, Ankam Rishwanth and Ravula Navya Sri

ABSTRACT

An accurate, precise, specific, simple and rapid RP-HPLC method was developed and subsequently validated for the determination of Nevirapine in API and Pharmaceutical dosage form. Better separation of the drug was achieved on Waters ODS (C18) RP Column, 250 mm x 4.6 mm. with a mobile phase consisting of a mixture of Methanol: Phosphate buffer in the ratio of 75:25 v/v at a flow rate of 1ml/min, with detection at 279nm using UV-Visible Detector. The developed method was validated for different parameters such as linearity, accuracy, precision, limit of detection (LOD), limit of Quantization (LOQ), robustness and the results were found to be within the limits according to ICH (International Conference on Harmonization) guidelines. The retention time of Nevirapine was found to be 3.462 min. The method was found to be linear in the range of 0- 14μg/ml with a correlation coefficient (r2) of 0.999. The LOD and LOQ of the method were calculated to be 0.003 and 0.009μg/ml respectively. The Precision was estimated by employing repeatability; intra-day and inter-day studies and the results were calculated as %RSD values and were found to be within the limits. The average recovery of the analyte was found to be within the limit which confirms the accuracy of the method.

Keywords: Nevirapine, RP-HPLC, Method Development, ICH Guidelines.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More