Photo Gallery

Login

Search

News & Updation

  • WJPPS APRIL ISSUE PUBLISHED
  • APRIL 2019 Issue has been successfully launched on 1 April 2019

  • WJPPS Impact Factor
  • Its our Pleasure to Inform you that WJPPS Impact Factor has been increased from 6.647 to 7.421 due to high quality Publication at International Level

  • ICV
  • WJPPS Rank with Index Copernicus Value 84.65 due to high reputation at International Level

  • Call for Paper
    • WJPPS  Invited to submit your valuable manuscripts for Coming Issue.
  • Updated Version
  • WJPPS introducing updated version of OSTS (online submission and tracking system), which have dedicated control panel for both author and reviewer. Using this control panel author can submit manuscript
  • Journal web site support Internet Explorer, Google Chrome, Mozilla Firefox, Opera, Saffari for easy download of article without any trouble.
  •  

Abstract

DEVELOPMENT AND VALIDATION OF A LC-MS/MS METHOD FOR THE DETERMINATION OF LETERMOVIR IN SPRAGUE DAWLEY RAT PLASMA AND ITS APPLICATION TO PHARMACOKINETIC STUDY

K. N. Rajanikanth, Dr. V. Kiran Kumar, Dr. VVSS. Appala Raju, Dr. Macharla Venkata Ramana, Dr. T. Shyam and Dr. N. Appala Raju*

ABSTRACT

During the preclinical study of the new antiviral drug, Letermovir, a sensitive liquid chromatography tandem mass spectrometry method was newly developed to study the pharmacokinetics of Letermovir in rat plasma and subcutaneous tissue samples. Although Letermovir was unstable in the rat plasma and subcutaneous tissue samples, pretreatment with EDTA and phosphoric acid (4 %) inhibited its degradation. The lower limits of quantification (LLOQ) for Letermovir were fully validated as 5 ng/ml in plasma and 5 ng/g in tissue with acceptable linearity, intra- and inter-assay precisions, and accuracy. Measurement of Letermovir concentration in plasma after intravenous injection via LC-MS/MS yields plasma concentration-time curves (AUC 0-∞) with areas of 667 ng•min/ml and an elimination half-life (t1/2) of 4.8 min. The concentration of Letermovir in the subcutaneous tissue samples was 13.1 μg/gm tissues at 30 minutes after a single dermal application (1 mg/ml, 50 μl) to a full-thickness excisional wound. Here, a highly sensitive and specific LC-MS/MS assay with a lower limit of quantification of 5 ng/ml was developed and validated to quantify Letermovir in rat plasma. This method is useful for pharmacokinetic studies of the peptide drugs in rats.

Keywords: LC-MS/MS, Pharmacokinetics, Plasma, Quantitation, Subcutaneous Tissue.


[Full Text Article]

Call for Paper

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Online Submission

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More

Email & SMS Alert

World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS)
Read More