Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY OF MUSHROOM PLEUROTUS OSTREATUS EXTRACT AGAINST EXTENDED SPECTRUM BETA LACTAMASE (ESBL) PRODUCING MICROORGANISMS

Dr. Debasmita Chatterjee*, Dr. Dipankar Halder, Dr. Satadal Das

ABSTRACT

Backrround: Extensive use of antibiotics, followed by non compliance of the patient resulted in the development of more than one drug resistant bacterium. They are responsible for rapid outbreaks and are primary cause for nosocomial infection. Extended spectrum beta lactamase (ESBL) positive strains are resistant to 3rd and 4th generation cephalosporins and monobactams. Aim: To study the antibacterial efficacy of the oyster mushroom Pleurotus ostreatus extract against six ESBL positive isolates. The detection of different phytochemicals constituent of the crude extract was also taken into consideration. Methods: The antibacterial efficacy of the oyster mushroom Pleurotus ostreatus was evaluated against six ESBL positive isolates via agar disc diffusion assay, minimum inhibitory concentration assay (MIC), minimum bactericidal concentration assay (MBC). The phytochemical composition of the crude extract was assayed by UV-Vis spectrophotometry. Results: Six isolates of ESBL producing bacteria were cultured from various clinical samples of patient after taking informed consent. The zone of inhibition of the extract of mushroom lies within 11mm – 12mm. MIC study have revealed the potent antibacterial efficacy of the mushroom extract against the ESBL positive isolates. The MBC values of the mushroom extract lies in the range 10 – 21mg/mL against the ESBL positive isolates. The mushroom extract was scanned in the range 200 – 800nm by UV-Vis spectrophotometry and it showed the presence of probable phytochemicals based on peak analysis. Conclusion: Oyster mushroom P. ostreatus showed potent antibacterial efficacy against ESBL producing bacteria. Therefore oyster mushrooms can be taken as dietary supplements along with our daily nutrition.

Keywords: Mushroom, ESBL producing microorganisms, antibacterial activity, UV-Vis spectrophotometry.