Abstract

ISOLATION, CHARACTERIZATION AND ANTIMICROBIAL SCREENING OF MDR-UTI PATHOGENS AND THEIR SUSCEPTIBILITY TO SECONDARY PLANT METABOLITES

Megha Sirohi* and Ayushi Chaudhary

ABSTRACT

Urinary tract infection (UTI) is one of the most common microbial infections affecting all age groups across the life span. The present study was aimed to gain knowledge about the type of pathogens responsible for urinary tract infections and evaluation of antimicrobial activity of various plant extracts against the pathogens. 11 samples were collected and processed. In that samples were confirmed as urinary tract infection. Bacterial species isolated from urine samples were Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus sp., From the UTI sample Shigella sp., are also isolated usually produce self-limited gastrointestinal infection that rarely result in extraintestinal complications. Present study showed that E.coli and Staphylococcus aureus isolates were the predominant pathogens. Among the tested sample various bacteria isolated from urine samples (88.4%) were sensitive to the 5 tested antibiotics i.e Vancomycin, Ceftazidine, ampicillin, Chloramphenicol, pyrodoxin. Vancomycin and chloramphenicol showed maximum activity against Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumonia, E.coli. The results also indicated that the most of urinary tract infection diseases were by Gram negative bacteria. Currently, the clinically available treatment is not effective against the antibiotic resistance developed by some bacterial species. However, plant-based antimicrobials have immense potential to bacterial, fungal, protozoal and viral diseases without any known side effects. Such plant metabolites include medicinal plants were selected to evaluate antimicrobial activity of methanol, ethanol, acetone and chloroform extracts against 7 bacterial isolates of major urinary tract pathogens by well diffusion method and MIC. The acetone extract of Foeniculumvulgare seeds, Punica granatum, Syzygium aromaticum, zingiber officinales, Psidium Guajava in which Foeniculumvulgare and Punica granatum showed maximum activity against E.coli, Bacillus Cereus, Staphyloccocus. The main purpose of the study was to identify the pathogens from urinary tract infection patients and detect the effectiveness of selected species of plant extracts against these pathogens.

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