Abstract

EFFECTS OF PURIFIED VENOM TOXINS FROM SPIDER CROSSOPRIZA LYONI ON HEMATOLOGICAL PARAMETERS IN ALBINO MICE

Ravi Kumar Gupta and Ravi Kant Upadhayay*

ABSTRACT

In the present study venom toxins from spider venom Crossopriza lyoni were subjected to purify on a Sepharose CL-6B 200 column. For visualizing hematological effects sub-lethal dose of purified toxins was administered in laboratory reared albino mice. The hemoglobin level in blood was significantly increased to maximum level i.e., 118% & 128% of the control at 10 hours of treatment of 40% and 80% of 24-h LD50 respectively. The number of red blood cells (RBCs) was decreased to 84% and 86% while numbers of the total white blood cells (WBCs) was increased to 136% and 133% of the control after 10 hours of treatment of 40% of 24-h LD50 respectively. The level of mean corpuscular hemoglobin (MCH) was increased to 120% and 113% of the control at 6 hours of treatment with 40% and 80% of 24-h LD50 of spider venom. Low molecular weight toxic peptides ranging from 14.3-63 kDa were identified in spider venom with strong cytotoxic action that caused intra-vascular hemolysis of RBCs, leucocytes, platelets and vascular endothelium. From comparison of gel chromatographs eluted toxins peptide molecular weight was ranging from 6.2-64 kDa.

Keywords: Spider venom, Crossopriza lyoni, gel filtration chromatography, hematological parameters.