Abstract

BIOGUIDED FRACTIONATION AND CHARACTERISATION OF THE EXTRACTS OF STROBILANTHES CILIATUS NEES

K. Shalini*, Shyni Bernard and K. Radha

ABSTRACT

Herbalism is based on relationship between plant and human, plant and planet, human and planet. Use of herbs in the healing process means taking part in an ecological cycle. The anti-inflammatory activities of Strobilanthes ciliatus was evaluated by several in vitro methods. The petroleum ether and alcoholic extracts of leaf and total alcoholic extract of stem was taken for evaluation. The anti-inflammatory activities were evaluated by cyclooxygenase inhibition assay, 5-lipoxygenase inhibition assay, myeloperoxidase, inducible nitric oxide synthase estimation, and cellular nitrite estimation on RAW 264.7 cell line. All the three extracts showed anti-inflammatory activity, but the petroleum ether extract of leaf showed more significant activity. Thus the promising extract (LPE) was subjected to column chromatography. The isolated compound was characterised by UV, IR, NMR, HRMS spectral studies and it was identified as Betulin. The COX and 5-LOX assay of the isolated compound was further conducted to justify and support the use of the plant as anti-inflammatory agent.

Keywords: Strobilanthes ciliatus, Anti-inflammatory, Column chromatography, Characterisation, Betulin.