Abstract

PHYTOCHEMICAL SCREENING, INHIBITION OF ANGIOTENSIN CONVERTING ENZYME ACTIVITY AND ANTI OXIDANT OF EXTRACT PANDAN WANGI LEAVES (PANDANUS AMARYLLIFOLIUS ROXB) IN VITRO

Shirly Kumala*

ABSTRACT

Pandan wangi leaves (Pandanus amaryllifolius Roxb.) generally only used by the Indonesians people as food additive. Empirically pandan wangi leaves has properties to reduce high blood pressure, and also have the antioxidant activity. To prove this statement a reseach was conducted by blocking the action of Angiotensin Converting Enzyme (ACE) the 70% ethanol extract pandan wangi leaves, using the method of Cushman and Cheung. Antioxidant activity with DPPH Methods. Phytochemical screening also conducted on the powder and 70% ethanol extract pandan wangi leaves and showed secondary metabolites i.e. flavonoids saponins, steroids / triterpenoids and coumarin. The activity results of the ACE inhibitor towards captopril, 70% ethanol extract of pandan wangi leaves, IC50 value of 10.17 ± 0.16 bpj; 28.55 ± 1.03 bpj respectively. Antioxidant activity was 70.678 bpj. The conclusion Pandanus amaryllifolius Roxb extract have antioxidant activity and also has the activity for inhibition of Angiotensin Converting Enzyme.

Keywords: Pandan Wangi Leaf, Angiotensin Converting Enzyme (ACE), DPPH.