Abstract

ISOLATION AND CHARACTERIZATION OF EIMERIA SPECIES IN POULTRY USING CONVENTIONAL METHODS AND COCCIMORPH PROGRAM IN KHARTOUM STATE, SUDAN

Eassa A. Eassa, El Ayis A. Abubaker* and Muna A. M. Khaier

ABSTRACT

An outbreak of Coccidiosis has a very high negative and economic impact on the flock as well as for the poultry producer as treatment alone cannot prevent the economic losses. This study was carried out in Khartoum State during the year 2018. Samples for isolation of field Eimeria species’ oocysts were collected from droppings and intestines of chickens from chicken flocks farms and Slaughter houses located in different localities of Khartoum State. Samples were taken from birds showing typical clinical signs of coccidiosis and not subjected for treatment. Eighty samples (45 faecal and 35 intestine samples) were collected aseptically from suspected birds from different localities of Khartoum State. Samples were subjected for parasitological and online Coccimorph identification system. Out of 80 samples collected from Khartoum State, 54 samples (67.5%) were positive for Eimeria Oocyst. Out of 50 samples collected from open rearing system, 33 samples (66%) were positive for Eimeria Oocyst. Out of 30 samples collected from closed rearing system, 21 samples (70%) were positive for Eimeria Oocyst. Out of 35 faecal samples collected for Eimeria Oocyst isolation, 28 samples (80%) were positive for Eimeria Oocyst. Out of 45 intestine samples collected for Eimeria Oocyst isolation, 26 samples (57.8%) were positive for Eimeria Oocyst. Out of 54 Eimeria Oocyst isolated from Khartoum State and subjected to convential methods and Coccimorph program characterization, the identified Eimeria spp. were: 21 Eimeria mitis (38.8%), 19 Eimeria necatrex (35.2%), 7 Eimeria tenella (13%) and 7Eimeria maxima (13%). This is the first report in Sudan of using Coccimorph system for identification of Eimeria spp. The isolates scored high probability percentages.

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