Abstract

IN-VITRO EVALUATION OF ANTI-INFLAMMATORY POTENTIAL OF NOVEL SIDDHA FORMULATION PAVAZHA PARPAM BY ALBUMIN PROTEIN DENATURATION ASSAY

J. Nisha*, N. J. Muthu Kumar, N. Anbu3 and V. Banumathi

ABSTRACT

Inflammation is biological response of the living being towards specific insults. Inflammation is characterized by pain, redness, swelling and dysfunction of the tissues and organs, is the normal result of host protective responses to tissue injury caused by numerous stimuli. Inflammation leads to the upregulation of a series of enzymes and signaling proteins in affected cells and tissues. The commonly used drugs for the management of inflammatory conditions are non-steroidal anti-inflammatory drugs (NSAIDs), which have several adverse effects especially gastric irritation leading to the formation of gastric ulcers. Herbs have been used in traditional medicine since many years and the use of herbal-derived natural products as a therapeutic tool has been increasing considerably. Herbs become the integral part of the siddha system of traditional medicine since it has unique feature of counteracting the oxidative stress there by preventing the generation of free radicals. It was evident through several research outcome that the oxidative stress worsen the condition of the inflammation by promoting synthesis and release of cytokines. Pavazha parpam (PP) is a novel siddha formulation as indicated for the treatment of bronchitis, tuberculosis, diarrhea etc as per the literature. But still now there is no documentary evidence on briefing the inflammatory potential of PP. Hence this study is the first of its kind attempting for in-vitro anti-inflammatory evaluation of the formulation PP using protein denaturation assay. The result obtained from the present study has clearly indicates that the test drug PP was effective in inhibiting heat induced albumin denaturation. Maximum percentage inhibition of about 38.41 % was observed at 500 μg/ml when compare to that of the Diclofenac sodium, a standard anti-inflammatory agent with the maximum inhibition 95.98 % at the concentration of 100 μg/ml.

Keywords: Inflammation, Herbal-derived, Siddha, Pavazha parpam, Anti-inflammatory, Diclofenac sodium.