EFFECT OF FRACTIONS OF BRIDELIA MICRANTHA ON INSULIN RESISTANCE AND LIPID PROFILES IN HIGH SALT-INDUCED INSULIN RESISTANT RATS AND IDENTIFICATION OF PHYTOCONSTITUENTS BY GC-MS
Ajao Folasade O* and Soladoye Ayodele O.
ABSTRACT
This present study investigates the effect of fractions of Bridelia micrantha leaves on insulin resistance and lipid profiles in high salt-induced insulin resistant rats and its identification of phytoconstituents by Gas chromatography-mass spectroscopy (GC-MS). Crude methanol leaf extract of Bridelia micrantha was fractionated using silica gel column chromatography to yield 48 fractions. Pooling together of fractions with similar thin layer chromatographic (TLC) mobility profile afforded four major fractions (Fr A – Fr D). Bioactivity of the fractions obtained was evaluated in insulin resistant Wistar rats.
Female Wistar rats were given (p.o.) normal diet (CON), high-salt diet (8%) daily (HS), Fr A-Fr D (HS rats treated with 10mg/kg bw of fractions A-D). Insulin resistance (IR) was estimated using the homeostatic model of assessment (HOMA). Results showed that high salt diet led to significant increases in insulin resistance, plasma insulin, total cholesterol (TC), triglyceride (TG), TC/HDL-cholesterol, and decreased glucose tolerance. Fraction B was the most effective in attenuating insulin resistance and dyslipidaemia. Fraction B of methanol extracts of Bridelia micrantha yielded 18 different phytochemicals confirmed by GC-MS analysis, some of which are known to have antidiabetic properties. In conclusion, the present study report the use of fractions of Bridelia micrantha leaves in the attenuating insulin resistance and dyslipidaemia in rats fed high salt diet and also identified the presence of compounds that could be responsible for these actions.
Keywords: Bridelia micrantha, High salt, Insulin resistance, Dyslipidemia, GC-MS.
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