Abstract

SEASONAL EFFECT ON EXTRACTION, QUANTITATION & PURITY ESTIMATION OF GENOMIC DNA FROM CASSIA ALATA L. LEAVES

Ribek Thapa, Baibhav Karki, Sudarshan Chettri, Tanaya Ghosh, Prasenjit Mitra, Prasanta Kumar Mitra*

ABSTRACT

Identification of a plant scientifically has its own importance. This is done by genetic study for which isolation of pure DNA from the plant source is essential. Cassia alata L.(C. alata L.) is a medicinal plant having several pharmacological properties. For its scientific identification isolation of genomic DNA from the plant is required. Objective of the present work was, therefore, isolation-quantitation and purity estimation of the genomic DNA from C. alata L. leaves. As season has effect on synthesis of chemicals in the plant parts, objective was also to study the seasonal effect on concentration of pure DNA in C. alata L. leaves. Leaves of C. alata L. were collected during autumn, winter, summer and rainy seasons, identified by experts and the leaves were processed for extraction, quantitation & purity estimation of genomic DNA by the conventional methods. Results showed that DNA was isolated from C. alata L. leaves in high amount during summer season (March – May). Isolated DNA was found pure. It is concluded that C. alata L. leaves of summer season may be used to get high amount of pure DNA.

Keywords: Seasonal effect, Cassia alata L., DNA extraction, Quantitation, Purity estimation.