Abstract

EVALUATION OF HEPATOPROTECTIVE POTENTIAL OF SIDDHA FORMULATION PUNGAMPOO CHOORANAM AGAINST PARACETAMOL INDUCED HEPATOTOXICITY IN ZEBRAFISH MODEL

J. Nisha*, N. Anbu, N. J. Muthu Kumar, V. Banumathi

ABSTRACT

Liver plays a versatile role on detoxification and metabolism of xenobiotics. This could be the main reason that liver often prone to inflammation and degeneration. Management of liver disease becomes a major public threat in the developing countries. Conventional medication available for treating liver disorders fails to provide adequate cure and further in some cases it even worsen the condition. Hence it’s a right time to explore alternate complimentary therapy for Indian system of traditional medicine such as siddha for effective management of clinical. Siddha medicines are pioneers in specific ailment for curative against liver disease since centuries. The main aim of the present investigation is to evaluate the hepatoprotective activity of the siddha formulation Pungampoo Chooranam (PPC) which majorly comprises of Pongamia pinnata in paracetamol induced liver dysfunction in zebrafish model. Induction of hepatotoxicity in Zebrafish was carried out by admixing paracetamol 5mM (755.8mg) per liter concentration. Zebrafish (Danio rerio) attains greater importance in the field reproductive biology due to it’s close genetically resemblance with respect to that of the humans. The results obtained from the present investigation has clearly reflects that the drug PPC at the concentration of 250 and 500 mg/liter has significantly ameliorated the Paracetamol induced hepatotoxicity in zebrafish model dose dependently. This study has provided an evidence based data on exploring the formulation PPC as a hepatoprotective agent for clinical management of liver disease.

Keywords: Liver, Siddha, Pungampoo Chooranam, Paracetamol, Zebrafish, Hepatoprotective activity.