Abstract

FOLIC ACID, B12, C AND E VITAMIN SUPPLEMENTATION REDUCES GENOMIC DAMAGE IN CHRONIC RENAL FAILURE PATIENTS UNDERGOING HEMODIALYSIS

Thatiane Lorena Miranda Batista, Jean Carlos Vencioneck Dutra*, Jean Moisés Ferreira, Esdras Andrade Santana, Cristiane Campostrini Cruz and Maria do Carmo Pimentel Batitucci

ABSTRACT

Chronic renal failure patients, especially those receiving hemodialysis, may present a higher frequency of DNA damage. This study was aimed to evaluate the effects of supplementation with folic acid, B12, C and E vitamins on the basal micronucleus frequency observed in lymphocytes and buccal mucosa cells in chronic renal failure patients. The sample included 16 patients [n=16] with chronic renal failure who were receiving 3 times a week hemodialysis. Vitamin supplementation was conducted for 60 days. Peripheral blood and epithelial buccal cells from the patients were collected before the treatment (day 0), 30 and 60 days after the treatment. Lymphocytes and buccal cells were used to evaluate the basal micronucleus frequency reduction by Cytome Assay. Vitamin supplementation reduced the basal micronuclei frequency in lymphocytes, reaching 57.50% protection of DNA damage, after 60 days of treatment, as well as a decrease in the frequency of apoptosis in cells of the buccal mucosa after 60 days of vitamin treatment. We hypothesize that vitamin supplement likely acts on mechanisms related to oxidative stress into the cells, reducing its cytotoxicity and genotoxicity. These findings suggest that the vitamin supplementation proposed in this study may help reduce cytotoxic and genotoxic damage in chronic renal patients.

Keywords: Renal chronic disease. Cytome Assay. Lymphocytes. Epithelial cells.