Abstract

SIMULTANEOUS DETERMINATION AND VALIDATION OF PIROXICAM AND RABEPRAZOLE IN COMBINED PHARMACEUTICAL DOSAGE FORM BY RP-HPLC METHOD

Manzoor Ahmed, Ramya C*, A Satish Kumar Shetty and G Narayana Murthy

ABSTRACT

A simple, rapid reverse phase High-performance liquid chromatographic method was developed and validated for the simultaneous estimation of Piroxicam (PIR) and Rabeprazole (RAB) in bulk and pharmaceutical dosage forms. Chromatography was carried out by using a Chromosil C-18, column having 250 × 4.6 mm internal diameter with a mixture of Acetonitrile and Phosphate buffer pH 6.8 in the ratio of 50:50 (v/v) as mobile phase. Determination of the different analytical parameters such as linearity, accuracy, precision, specificity, limit of detection (LOD) and limit of quantification (LOQ) was done. The calibration curve was found to be linear for each analyte in the desired concentration range. The % recovery was found to be 100.15 and 99.47 for Piroxicam and Rabeprazole respectively. The proposed method is highly sensitive, precise and accurate, which was evident from the LOD value of 9.99 and 11.09 ppm for Piroxicam and Rabeprazole respectively and hence the present method can be applied successfully for the quantification of active pharmaceutical ingredient (API) content in the combined formulations of Piroxicam and Rabeprazole.

Keywords: Piroxicam, Rabeprazole, RP-HPLC, Method development, Validation, 260 nm.