ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS ESTIMATION OF CATECHIN AND CURCUMIN BY HPLC IN AN AYURVEDIC FORMULATION
Shraddha Joshi*, Dr. Varsha M. Jadhav and Dr. Vilasrao J. Kadam
ABSTRACT
A simple, selective, particular and reproducible Reverse phase HPLC
technique has been developed for the simultaneous estimation of
Catechin and Curcumin from ayurvedic formulation. The method was
validated using parameters such as linearity, specificity, precision,
accuracy, limit of quantification, limit of detection and robustness as
per ICH guidelines. The method was carried out on a Hemochrom
Intsil [(C18 (250 X 4.6 mm, 5 μm)] column with a mobile phase
consisting of methanol: 0.1% orthophosphoric acid at a flow rate of 0.8
ml/min. Detection was carried out at 269 nm. The retention time of
Catechin and Curcumin was 3.169 and 7.669 min, respectively. The
regression analysis data indicated good linear relationship for the calibration plots for
Catechin and Curcumin in the range of 50-110μg/ml and 2-14 μg/ml and regression
coefficient was 0.993 and 0.992 respectively. The proposed method can be used for the
estimation of these markers in combined ayurvedic formulation.
Keywords: Catechin, Curcumin, Ayurvedic formulation, Simultaneous estimation, Analytical method validation, HPLC.
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