Abstract

IN VITRO REGULATION OF ADIPOGENIC DIFFERENTIATION OF ADIPOSE-DERIVED MESENCHYMAL STEM CELLS

Shaymaa H. El-Arabi, Mohamed A. Sobh, Ahmed M. El-Waseef and Amr Negm*

ABSTRACT

Human adipose tissue acts as a practical supply of mesenchymal stem cells (hAMSCs) compared to other donor sites. So our attention was directed to study the adipogenic differentiation capacity of mesenchymal stem cells derived from human adipose tissue. Extraction of MSCs from human adipose tissue were done. After that we were studied the in vitro characterization of MSCs clustar differentiation(CD) surface marker. Moreover, we tested the ability of it to form fibroblast-like colony and differentiated into adipogenic tissues by special identification markers. Some identification tests have been done such as: viability test, Fibroblast-Like Colony- Forming Unit Assay and CD surface marker to assure that we have correctly obtained mesenchymal stem cells. These hAMSCs expressed CD29, CD 90, CD 105 and CD13 but not CD14 and CD34. RT-PCR of adipogenic differentiated hAMSCs expressed Lipoprotein Lipase, Leptin , Adiponectin, peroxisome proliferator-activated receptor-γ and a transcription factor known to be involved in control of adipocytic differentiation at different time intervals after 4, 7, 14 and 21 days. HAMSCs have the ability to proliferate into monolayer culture and multilineage adipogenic differentiation as a result of treating with inductive conditions and thus have potential clinical applications in regenerative medicine.

Keywords: Human Adipose Tissue, Mesenchymal Stem Cells, Surface Marker, Differentiation, Adipogenesis and Gene Expression.