Abstract

ISOLATION AND CHARACTERIZATION OF STIGMASTEROL FROM METHANOLIC EXTRACT OF RHIZOMES OF ALPINIA CALCARATA

P. Pratheema*, S. Gurupriya, K. Sahira Banu and L. Cathrine

ABSTRACT

Objective: The objective of this study was to isolate and characterize the stigmasterol from the methanolic extract of rhizomes of Alpinia calcarata. Methods: The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis. Purity was checked by high performance thin-layer chromatography (HPTLC) and high performance liquid chromatography (HPLC) technique. Results: The infra-red spectra showed specific absorption bands for stigmasterol viz.1641.74 cm-1 (C=C absorption peak), 1453.03 cm-1 (CH2), 1056.06 cm-1, 1370.01 cm-1 CH2(CH3)2., 1056.06 cm-1 (cycloalkane). Structural elucidation of stigmasterol was done by spectrum analysis such as 13C and 1H depth nuclear magnetic resources. Mass spectra of stigmasterol showed a parent molecular ion [M+] peak at m/z 412 which corresponds to molecular formula C29H48O. Conclusion: From the spectral characteristics, the isolated compound from the methanoilic extract of Alpinia calcarata rhizomes was confirmed to be stigmasterol.

Keywords: Stigmasterol, Alpinia calcarata, Isolation, Rhizomes.