T-MAZE ALTERNATION FOR ZEBRAFISH BEHAVIOURAL STUDIES
Jatin Prakash, Chaitra Mandadhi, Guruprasad*
ABSTRACT
There is growing interest in using zebrafish (Danio rerio) as a model of
neurodegenerative disorders such as Alzheimer's disease. This protocol
details a new T-maze to assess the cognitive ability for zebrafish. The
purpose of the T-Maze is to assess spatial memory, behavior and
learning of zebrafish in a control with disease model/intervention
group, by observing their ability to remember which arm they have
previously entered. The T-maze we designed is an open apparatus in
the form of a T placed horizontally, it consists of a capital T-shape
maze. Each arm, including the starting arm, ranges from (17) Inc in
length to accommodate zebrafish. The width of the pathways is
approximately (2.5) inc. left goal arms contain a well with food reward and the right arm
containing a dc motor with a rotating fan fixed to it. This development of new methods for
high-throughput tests of cognitive function for fish can provide means for rapid screening of
potential toxic agents as well as promising therapeutic agents.
Keywords: zebrafish, dc motor, spatial memory.
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