Abstract

POST-TREATMENT AUGMENTATION OF ANGIOGENESIS AND ANTI-OXIDATIVE INDEX OF ADULT STEM CELLS DERIVED FROM WHARTON’S JELLY

Ghazanfer Ali Nasir, Sana Javaid Awan*, Ayesha Tariq, Ammad Tanvir, Tahir Maqbool, Saba Boota, Somia Shehzadi, Fahim Hadi, Samra Javed, Umair Mohsin, Mashal Masud

ABSTRACT

Objectives: The umbilical cord is proven as a potential source of adult stem cells, due to its abundance, harvesting, deficiency of ethical issues and wider potential of differentiation. Mesenchymal stromal cells (MSCs) derived from umbilical cord Wharton's jelly (WJ) are called as Wharton’s jelly mesenchymal stromal cells (WJMSCs). Chronic kidney diseases (CKD) are categorized by functional loss and progressive damage of the parenchymal tissue of kidney which results into chronic kidney failure. Present study was planned to examine the potential role of WJ-MSCs for angiogenesis, wound healing and anti-oxidative index after their pre-treatment. Methods: The treatment of 2nd passage WJ-MSCs was done by using serum from CKD patients (unhealthy serum) and normal humans (healthy serum). To estimate the vascular endothelial growth factor (VEGF) levels in various treatment groups sandwich ELISA and immunocytochemistry was done. Wound healing capacity and anti-oxidative enzymes were performed independently groups. Anti-oxidants were projected by SOD, CAT, APOX and GSH assay. Results: VEGF levels are found to be increased in post-treatment group. Moreover, improved angiogenesis and enhanced wound healing expression show a reduction in injured group (post-treatment group). Whereas increased levels of anti-oxidative enzymes in post-treatment group result in decrease of reactive oxygen species (ROS). Conclusion: In conclusion our study reveals that serum treatment significantly enhanced the repair potential of WJMSCs by its anti-oxidative and angiogenic capacities. The study emphasizes the efficacy and feasibility of serum treated WJMSCs and may be promising in its application and transplantation in CKD in vivo.

Keywords: Mesenchymal stromal cells, chronic kidney disease, angiogenesis, wound healing, Wharton’s jelly mesenchymal stromal cells.