Abstract

IN VITRO EVALUATION OF ANTIOXIDANT POTENTIAL AND IN VIVO STUDIES FOR PRECLINICAL TOXICITY WITH HEPATOPROTECTIVE ACTIVITY OF SAFOOF-E-AKSEER-E-JIGAR

Najeeb Khan M.*, Touheed Ahmed S., Waheed M. A., Viquar Uzma, Yousuf Ali Mir, Tariq S., S. S. Y. H. Qadri, Shaffin Azra, Naseeb Fouzia, Zehra Amtul, Rasheed N. M. A and Parveen N.

ABSTRACT

To Evaluate the in vitro and in vivo antioxidant potential and hepatoprotective activity of Safoof-e-Akseer-e-Jigar (SAJ). The antioxidant study of SAJ was carried out in vitro by using 1,1-diphenyl-2-picryldrazyl (DPPH) free radical scavenging method. Acute oral toxicity was evaluated in Swiss albino mice to determine the maximum Tolerated Dose (MTD). Hepatoprotective activity was evaluated by paracetamol induced liver injured animal model in Wistar rats. Acute oral toxicity study revealed that SAJ is a safe compound as none of the animals died during the observation period and there were no sign and symptoms of toxicity related to it and its MTD>2600mg/kg in mice indicating its high safety profile. SAJ at a dose of 180mg/kg, was found to have significant (p<0.05) hepatoprotective activity by the inhibition of elevated biomarkers of paracetamol induced liver damage viz., SGOT, SGPT, ALP, and SB apart from maintaining normal haemoglobin levels and was found to be comparable with that of Liv 52 which was confirmed by the histopathological examination of liver of rats and also revealed on pathological changes even after administration of SAJ for 7 days. The present studies demonstrate the potential Anti-oxidant and Hepatoprotective properties of Safoof-e-Akseer-e-Jigar.

Keywords: Safoof-e-Akseer-e-Jigar (SAJ), Antioxidant, Hepatoprotective.