Abstract

FORMULATION AND IN-VITRO CHARACTERIZATION OF BIODEGRADABLE ISONIAZID NANOPARTICLES IN COW URINE

Vothani Sarath Babu*, C. Pallavi and I. Sowkar Baig

ABSTRACT

Scientific community believes that Nanotechnology offers new ways to address residual scientific concerns for Mycobacterium Tuberculosis (TB). Nanoparticles one of the great colloidal drug delivery as controlled drug deliver as well as site specific delivery. Nanoparticles acquire an exclusive stability then compared to other novel drug delivery system. This phenomenon may be help to targeting organs. Isoniazid Nanoparticles were Prepared by using of biodegradable polymer mostly compatibility to the human system. The present study was carried to prepare Isoniazid nanoparticles showed significant advancement of this technology is that the using of nanoparticles as drug carriers are had high stability and carrier capacity the sustained drug release from the matrix, such properties of nanoparticles enables improvement of drug bioavailability and reduction of the dosing frequency. The aim of the present study was to develop and evaluate nanoparticulate drug delivery system of highly cow urine soluble drug Isoniazid by using sodium alginate polymer were prepared by two techniques i.e. cross-linking method and solvent evaporation method. Sodium alginate gel was prepared using cow urine. Sodium alginate was used as a poly anionic natural polymer. The nanoparticles were prepared using different ratios of sodium alginate and isoniazid in the ratio of (1:1,1:2and1:3). The encapsulation efficiency was also studied to find out the percentage drug entrapped in the prepared isoniazid Nanoparticles. The result of ratio 1:1 (isoniazid:aliginate) showed a good encapsulation efficiency of 89.5%.Isoniazid nanoparicle was confirmed by FT-IR,DSC and quantitated by prepared nanoparticle appeared spherical with a dense drug core in transmission electron microscope studies., mean diameter of nanoparticle was 560.3 nm, and the zeta potential was -0.4mev. The drug loading decreased with increasing polymer concentration. The in-vitro dissolution studies were carried out for nanoparticles by dialysis method. All the nanoparticles formulations were able to sustained diffusive drug release was observed in vitro, following zero order kinetics releasing the drug play load for a period of 16hrs. Prepared isonized Nanoparticle in sodium alginate provided sustained released. The Nanoparticle technique develop can be a good choice for the development of sustained antituberculosis drug carrier.

Keywords: Isoniazid, Nanoparticle, Cow urine, Sodium Alginates.